Signalway Antibody has developed an unique monoclonal antibody technology which is named as
Recombinant Monoclonal and Single B Cell Antibody (RmSab™) Technology. This technology
employs a whole set of optimized methods including single B cell sorting, enriching and proliferation,
applications screening and validation, and gene cloning, sequencing and expression. It is inaccessible
to traditional hybridoma technology, and is faster with high success rate and high antibody affinity
compared to general single B cell sorting, enriching and sequencing technology.
This revolutionary method has successfully been applied to develop high quality recombinant rabbit
monoclonal antibodies as well as other hosts. Our mission is to promote personal health and advancing
disease diagnosis by providing superior monoclonal antibodies to our customers in the research and
diagnostic fields.

Superior specificity

The unique characteristics of our RmSab™ may account for the improved quality of the antibodies
produced in rabbits. The high specificity of Rabbit Monoclonal Antibodies has clear edges in assays.

High affinity

Typically, Rabbit Monoclonal Antibodies have 10 – 100 times higher affinity than mouse monoclonal
antibodies. On average, the KD values of Rabbit Monoclonal Antibodies range from 10-10 up to 10-12M.

Improved sensitivity

Increased assay accuracy with decreased false positive rate.

 

Lower assay noise

Ideal choice for various application especially in IHC.

 

Lot-to-lot consistency

Lot-to-lot consistency due to recombinant technology.

Great Stability

Rabbit IgG has extra disulfide bonds in the variable region of the heavy chain. An extra disulfide bond
also exists between Vκ and Cκ. These extra disulfide bonds may result in the great stability and long
shelf life of rabbit antibodies.

 

Broad epitope recognition

Rabbit antigen presenting cells (APC) can present a broader range of epitopes than their mouse counterparts.
Rabbits are able to generate high affinity antibodies against difficult epitopes, such as protein modifications,
enzyme cleavage sites, or conformational epitopes as well as many less abundant proteins.

 

Antibody diversity

Rabbit antibodies recognize a greater diversity of epitopes per antigen compared to murine species. Rabbit
monoclonal antibodies can detect antigens at the picomolar range with high specificity and provide higher
signal intensity in a wide range of applications.Though strict screening and validation, we can provide
antibodies in various requirements including some functional applications.

 

Animal origin-free formulations

Due to production by cell culture.


 

With Recombinant Monoclonal and Single B Cell Antibody (RmSab™) technology platform,
Signalway Antibody offers recombinant monoclonal antibody development service.
We have successfully completed many satisfactory custom projects. We provide the following services.
Please contact us: order@signalwayantibody.com

Hosts

Rabbit (typically)	Mouse	Goat	Alpaca

 

Rabbit Monoclonal Service Provides

• One-stop service – from antigen to antibody validation.
• Flexible optimization at each stage.
• Both monoclonal antibodies and polyclonal antibodies if requested.

Highlights

• High affinity
• Superior specificity
• Broad epitope recognition including small molecules and post translational modifications
• Multiple applications for choice including WB, IF, IHC, ELISA, FCM, IP and more

 

Rabbit Monoclonal Antibody Service Process

Stage	Process	Duration
1	Expression plasmid construction	1.5 weeks
2	Immunogen production	2 weeks
3	Immunization	8 weeks
4	Final boost and B cell isolation	1.5 weeks
5	IgG gene cloning and expression validation	1.5 weeks
6	Recombinant antibody production and validation	1.5 weeks

Rabbit monoclonal antibody services will be typically completed within 4 months. If you have theongoing project,
please contact us on how to provide the fresh spleen or peripheral blood to us for the B-cell isolation.

Deliverables

• Five clones.
• Full length heavy & light chain genes in a non-proprietary plasmid.
• 100ug purified antibodies.
• Validation reports.

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